By Catherine Cooper
A suite of vintage and state of the art strategies of excessive application in answering particular organic questions on amino acids. universal equipment comprise these in response to HPLC or fuel chromatography separation and research after precolumn derivatization. New options in line with capillary electrophoresis separation, high-performance anion trade chromatography, and mass spectrometry also are provided. every one procedure is defined in step by step aspect to make sure winning experimental effects and emphasizes pattern practise, fairly the gathering and garage of physically fluids. updated and hugely functional, Amino Acid research Protocols bargains analytical and scientific chemists, in addition to a large diversity of organic and biomedical investigators, a wealthy compendium of laboratory instruments for the efficient research of either universal and unusual amino acids.
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Extra resources for Amino acids analysis Protocols
And Wilson, K. J. (1989) A comprehensive approach to amino acid analysis, in Techniques in Protein Chemistry (Hugli, T. ). Academic, London and New York, pp. 284–294. 2. Instructions for UltraMicro spin column, Amika Corp. 3. West, K. A. and Crabb, J. W. (1992) Application of automatic PTC amino acid analysis, in Techniques in Protein Chemistry III (Angeletti, R. ), Academic, London and New York, pp. 233–242. Purification of Proteins for AAA 37 4. , Mische, S. M. (1992) Amino acid analyis of PVDF bound proteins, in Techniques in Protein Chemistry III (Angeletti, R.
A common source of amino acid contamination is fingerprints. Be sure to wear gloves to keep this type of contamination away from the samples. Another source of contamination is dust. 2. Every time a sample is handled, a portion is lost. Minimize sample handling as much as possible. 3. It is important to choose the appropriate size of microspin column for your sample volume. For sample volumes of 5–25 µL, the smaller ultramicrospin columns are most appropriate. They have a packed bed volume of about 100 µL.
Kochhar, S. and Christen, P. (1989) Amino acid analysis by high-performance liquid chromatography after derivatization with 1-fluoro-2, 4-dinitrophenyl-5-L-alanine amide. Anal. Biochem. 178, 17–21. 3. Kochhar, S. and Christen, P. (1988) The enantiomeric error frequency of aspartate aminotransferase. Eur. J. Biochem. 175, 433–438. 4. , and Hudecz, F. (1988) Application of Marfey’s reagent in racemization studies of amino acids and peptides. J. Chromatog. 444, 115–122. 5. , Manning, J. , et al. (1989) Stereospecificity of reactions catalyzed by bacterial D-amino acid transaminase.