Download An Introduction to Genetic Engineering (Studies in Biology) by Dr Desmond S. T. Nicholl PDF

By Dr Desmond S. T. Nicholl

ISBN-10: 0521004713

ISBN-13: 9780521004718

Des Nicholl offers a brand new, totally revised, and improved variation of his well known undergraduate-level textbook. The publication keeps the various positive aspects of the unique variation and nonetheless bargains a concise technical creation to the topic of genetic engineering. it truly is divided into 3 major sections: easy molecular biology, equipment of gene manipulation, and sleek functions of genetic engineering. functions lined within the booklet comprise genomics, protein engineering, gene remedy, cloning, transgenic animals and crops, and bioethics. An advent to Genetic Engineering is vital interpreting for undergraduate scholars of biotechnology, genetics, molecular biology, and biochemistry.

Show description

Read or Download An Introduction to Genetic Engineering (Studies in Biology) PDF

Best molecular biology books

Horizontal gene transfer

The second one variation of Horizontal Gene move has been prepared to supply a concise and up to date assurance of crucial discoveries during this interesting box. Written through the main famous gene move and genome analytical scientists, this ebook information experimental proof for the phenomenon of horizontal gene move and discusses additional proof supplied by means of the hot crowning glory of genomic sequences from Archea, micro organism, and Eucarya participants.

Ion Channels Part B

This quantity plus its better half, quantity 294, provides innovative ideas for the learn of ion channels, starting from the molecular to the physiological point. those volumes complement quantity 207 of equipment inEnzymology Key gains* meeting* Genetics* Electrophysiology* Expression structures* version Simulations

Origin and Continuity of Cell Organelles

The 1st quantity of the sequence, on "The balance of the Differentiated nation" obtained many favorable experiences from the clinical neighborhood. Many readers appear to trust us that e-book of topical volumes is a helpful substitute to periodic compilations of really unrelated, notwithstanding up to date reports.

Additional info for An Introduction to Genetic Engineering (Studies in Biology)

Sample text

Current recommendations from the International Union of Biochemistry (IUB) and the International Union of Pure and Applied Chemistry (IUPAC) favour the terms coding/noncoding, with the coding strand of DNA taken to be the mRNA-like strand. This convention will be used in this book where coding function is specified. The terms template and non-template will be used to describe DNA strands when there is not necessarily any coding function involved, as in the copying of DNA strands during cloning procedures.

E. the measure of how radioactive the molecule is. For tracing purposes, a low specific activity will suffice but for probes, a high specific activity is necessary. In probe preparation the radioactive label is usually the high-energy ␤-emitter 32P. Some common methods of labelling nucleic acid molecules are described below. 1 End labelling In this technique the enzyme polynucleotide kinase is used to transfer the terminal phosphate group of ATP onto 5Ј-hydroxyl termini of nucleic acid molecules. If the ATP donor is radioactively labelled, this produces a labelled Radiolabelling of nucleic acids 31 Fig.

A) A primer is annealed to a single-stranded template and (b) the Klenow fragment of DNA polymerase I used to synthesise a copy of the DNA. A radiolabelled dNTP (often [␣-35S]dNTP, filled circles) is incorporated into the DNA. (c) Chain termination occurs when a dideoxy nucleoside triphosphate (ddNTP) is incorporated. (d) A series of four reactions, each containing one ddNTP in addition to the four dNTPs required for chain elongation, generates a set of radiolabelled nested fragments. The generation of fragments for dideoxy sequencing is more complicated than for chemical sequencing, and usually involves subcloning into different vectors.

Download PDF sample

Rated 4.46 of 5 – based on 21 votes