Download Bioluminescence and Chemiluminescence Part B by Nathan P. Colowick, Nathan P. Kaplan, Marlene A. DeLuca, PDF

By Nathan P. Colowick, Nathan P. Kaplan, Marlene A. DeLuca, William D. McElroy

ISBN-10: 0121820335

ISBN-13: 9780121820336

The significantly acclaimed laboratory typical, Methods in Enzymology, is without doubt one of the so much hugely revered courses within the box of biochemistry. considering 1955, every one quantity has been eagerly awaited, usually consulted, and praised through researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate this present day - really an important e-book for researchers in all fields of existence sciences

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18 BIOLUMINESCENCE [2] early date highlighted the fact that bacterial ATP turns over rather quickly. ). 45-47 In general, many bacteria have ATP levels around 1 fg (I0 -~5 g) per cell. Yeasts have around 100 times more and many animal cells contain around a picogram ATP (10 -~2 g). Some actual values are given in refs. 1-3. I will now consider, albeit briefly, the main extractants and give a few references wherein details can be obtained. 1. Boiling buffer, usually Tris-Cl, with EDTA; marine microbial sampies, 2 freshwater microbial samples/8 nematodes, 49 yeast, 5° rumen con- tents, ~2 mycoplasmas, 23 mycobacteria.

W. Chappelle and G. V. Levin, Biochern. Med. 2, 41 (1968). 5 A. Thore, S. Ansehn, A. Lundin, and S. Bergman, J. Clin. Microbiol. 1, 1 (1975). 6 A. Thore, A. Lundin, and S. Ansehn, J. Clin. Microbiol. 17, 218 (1983). [3] DETECTION OF BACTERIUREA 25 likely were inhibited by antimicrobial agents present in the urine. 7 The early study by Thore et al. 5 in fact reported 4% of samples tested were falsely negative, while 30% were falsely positive. ,8 using an ATP concentration limit of 6 × 10-14 M ATP as representative of 105 CFU/ml, found 7% false-negative samples and 27% false-positive samples.

Environ. Microbiol. 46 533 (1983). 13 p. C. T. Jones, J. Theor. Biol. 34, l (1972). 14 j. S. Franzen and S. B. Binkley, J. Biol. Chem. 236, 515 (1961). 15 L. Gustafsson, Arch. Microbiol. 120, 15 (1979). 16 M. Ohmori and A. Hattori, Arch. Microbiol. 117, 17 (1978). 17 A. Lewenstein and R. Bachofen, Arch. Microbiol. 116, 169 (1978). 18 C. M. M. Franco, J. E. Smith, and D. R. Berry, J. Gen. Microbiol. 130, 2465 (1984). 19 M. B. Nair and R. R. Eady, J. Gen. Microbiol. 130, 3063 (1984), 2o R. E. Strange, H.

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